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This section includes selected coursework-based projects, short-term academic training, and internship research.
Detailed information on my primary research is presented separately under Fern Biology and Development
PROTEIN STRUCTURE VISUALIZATION USING PYMOL
PROTEIN STRUCTURE VISUALIZATION USING PYMOL
During the time I participated the Sakura 2022 for the Short-course related to medicine at Tsukuba University, Japan. I have learned this technique in order to compare the protein structure, mutagenesis by using Pymol.My team at Tsukuba also have prepared a small project with the purpose to visualize the SAR-CoV 2 protein structures and define the difference between Delta and Omicron variants, then, We could design vaccines and drugs to treat the omicron variants.... Our small project was instructed by Dr. Kiong Ho at Tsukuba ...During the project, we have done:
- Collect the structure of COVID19 by PDB ( Protein Data bank)
- Using Pymol to visualize the structure [Pymol is a famous tool to visualize 3D molecular structures which is free and everyone can easy to download it...]
- Defined the reason why Omicron has stronger interactions with human receptor
- The structure is able to be applied for the mutagenesis technique to find medicine or vaccine...
Antioxidant activity
Antioxidant activity
The DPPH assay is used to predict antioxidant activities based on the mechanism by which antioxidants inhibit lipid oxidation, resulting in DPPH radical scavenging and thus determining free radical scavenging capacity.
Rotary evaporator
Aqueous extract
Collection the extract
DPPH assay
MOLECULAR BIOLOGY
MOLECULAR BIOLOGY
Our team have prepared the presentation which is about the Regulation of gene expression at the RNA level ...
Currently, I'm working as an undergraduate research intern at the Department of Plant-Biotechnology and Biotransformation...
I have prepared Chemical, cultural mediums, and done some experiments...
Plant tissue culture
Plant tissue culture
This is the plant-tissue culture practice at the Department of Plant-biotechnology and biotransformation
Preparation and sterilization: It depends on your purpose the components may be different, here is what I used
MS medium:
Skoog I (100ml/1L medium)
Skoog II (2.5 ml/1L)
Skoog III (5ml/L)
Glycine (2mg/mL)
MyO- Inoaitol (50mg/ml)
Sucrose
Agar...
Adjustment of the medium's pH: Really careful to control the pH by adding HCl or KOH solution ...
Sterilizing plant sample: It's a secret protocol that My project used to optimize the quality... However, as usual, We use Calcium hypochlorite or javel with a different ratio.
Plant sample is transferred to the medium and this task has to be done by working with Fume Hood and Biosafety Cabinets.
Growing plants is the process that needs you to observe and check to find the optimal conditions for plants to growth
SHOOT REGENERATION FROM LEAVES EXPLANTS
SHOOT REGENERATION FROM LEAVES EXPLANTS
The project is to evaluate the effect of physiological status on the capacity of bud formations in Chrysanthemum by using NAA and BAP.
Preparation of explants: Eight-week-old in vitro Chrysanthemum plants were cultured on MS medium S.
Determination of shoot regeneration: The first to sixth leaves from the apical bud were removed from the stem and wounded across the central veins using a culture knife. The leaves were cultured on MS medium and MS medium supplemented with 1.5 mg/l NAA and 0.5 mg/l BA. The cultures were maintained at 22 ± 2 °C, 3000 lux, and 65 ± 5% relative humidity. The number of shoots that regenerated from different positions on the leaves was observed and recorded after four weeks of culture, with four replicates.
Callogenesis of the leaves at the cutting site after 3 cultured weeks, it was observed the initial bud formation.
The cutting leaves on the basal MS medium
The cutting leaves on the basal MS medium
Leaves at different positions on a plant have different bud formation capacities due to differences in leaf size, growth rate, and hormone levels. Leaves with larger surface areas, faster growth rates, and higher hormone levels are more likely to form buds
The shoots regeneration of leaves at position of 3th and 4th
The shoots regeneration of leaves at position of 3th and 4th
The shoots regeneration of leaves at position of 5th and 6th
The shoots regeneration of leaves at position of 5th and 6th
Analysis of phytohormones of in. vitro sprouts from bean Cora white seeds (Phaseolus vulgaris L.)
Analysis of phytohormones of in. vitro sprouts from bean Cora white seeds (Phaseolus vulgaris L.)
Common bean (Phaseolus vulgaris L.) is not one of the most significant agricultural legumes in the world but also as a potential function foods that components is rich of primary and secondary metabolites source such as protein or phenolic and flavonoids compounds (Chávez-Mendoza & Sánchez, 2017).
Plant growth regulators (PGRs) are extracted from plants using methanol as the solvent. The extract is then purified using solvent partitioning and paper or thin-layer chromatography. The identity of the PGRs is confirmed by comparing their Rf values to known standards. In this study, the PGR activities of auxin (IAA), cytokinin (Zeatin), and gibberellin (GA3) will be analyzed.
Effect of pH on the production of α-amylase by Bacillus subtilis
Effect of pH on the production of α-amylase by Bacillus subtilis
The A-amylase production is upon on pH as one of the crucial factors
A-amylase activity varies from strain to strains in B.subtilis
The production of α-amylase by Bacillus subtilis strains can be optimized by tuning cultural media components such as pH, temperature, substrate concentration, and incubation period. Therefore, this project aims to determine the optimal pH for α-amylase production by Bacillus subtilis strains provided by the Department of Biochemistry.
B.SUBTILIS STRAIN IS POTENTIAL FOR A-AMYLASE PRODUCTION AT LARGE-SCALE INDUSTRIAL.
DEFINE THE MECHANISM OF THE CHANGE OF Ɑ-AMYLASE ACTIVITY AND Α-AMYLASE PRODUCTION RELATED TO EXTREMELY ACIDIC OR ALKALINE PH CONDITIONS.
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